sem and eds spectroscopy layered elemental mappings Search Results


energy dispersive spectrometer  (Oxford Instruments)
99
Oxford Instruments energy dispersive spectrometer
Energy Dispersive Spectrometer, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/Azteclive/pmc12844952-36-19-26
Average 99 stars, based on 1 article reviews
energy dispersive spectrometer - by Bioz Stars, 2026-09
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hif 1α  (Novus Biologicals)
97
Novus Biologicals hif 1α
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Hif 1α, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/HIF-1+alpha+Antibody/pmc05510229-128-39-40
Average 97 stars, based on 1 article reviews
hif 1α - by Bioz Stars, 2026-09
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energy dispersive x ray spectroscopy  (JEOL)
99
JEOL energy dispersive x ray spectroscopy
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Energy Dispersive X Ray Spectroscopy, supplied by JEOL, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/JEM-2100F+Transmission+Electron+Microscope/10__1007_slash_s13201___021___01458___6-58-5-9
Average 99 stars, based on 1 article reviews
energy dispersive x ray spectroscopy - by Bioz Stars, 2026-09
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wavelength dispersive spectroscopy wds element distribution maps  (JEOL)
97
JEOL wavelength dispersive spectroscopy wds element distribution maps
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Wavelength Dispersive Spectroscopy Wds Element Distribution Maps, supplied by JEOL, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/JXA-8230+Electron+Probe+Microanalyzer/10__1007_slash_s00126___016___0692___6-39-13-21
Average 97 stars, based on 1 article reviews
wavelength dispersive spectroscopy wds element distribution maps - by Bioz Stars, 2026-09
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iodine atoms  (Gatan Inc)
96
Gatan Inc iodine atoms
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Iodine Atoms, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/EDAX+Elite+T+EDS+System/pmc03216570-116-5-19
Average 96 stars, based on 1 article reviews
iodine atoms - by Bioz Stars, 2026-09
96/100 stars
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quantum gif energy filter  (Gatan Inc)
98
Gatan Inc quantum gif energy filter
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Quantum Gif Energy Filter, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/GIF+Continuum+and+Continuum+S/pmc08623765-168-10-9
Average 98 stars, based on 1 article reviews
quantum gif energy filter - by Bioz Stars, 2026-09
98/100 stars
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edax elemental mapping  (Gatan Inc)
96
Gatan Inc edax elemental mapping
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Edax Elemental Mapping, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/Element+EDS+System/10__1063_slash_5__0265752-36-32-32
Average 96 stars, based on 1 article reviews
edax elemental mapping - by Bioz Stars, 2026-09
96/100 stars
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oim analysis 7 3 software package  (Gatan Inc)
96
Gatan Inc oim analysis 7 3 software package
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Oim Analysis 7 3 Software Package, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/OIM+Analysis/10__1016_slash_j__addma__2022__103173-86-11-10
Average 96 stars, based on 1 article reviews
oim analysis 7 3 software package - by Bioz Stars, 2026-09
96/100 stars
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energy dispersive spectrometer eds mapping images  (JEOL)
99
JEOL energy dispersive spectrometer eds mapping images
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Energy Dispersive Spectrometer Eds Mapping Images, supplied by JEOL, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/JSM-7800F+Scanning+Electron+Microscope/10__1016_slash_j__apt__2021__09__027-60-14-24
Average 99 stars, based on 1 article reviews
energy dispersive spectrometer eds mapping images - by Bioz Stars, 2026-09
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nanosims 50 l ion microprobe  (CAMECA Inc)
90
CAMECA Inc nanosims 50 l ion microprobe
( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, <t>HIF-1α</t> and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.
Nanosims 50 L Ion Microprobe, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/nanosims+50l+ion+microprobe/pmc07347930-199-13-18
Average 90 stars, based on 1 article reviews
nanosims 50 l ion microprobe - by Bioz Stars, 2026-09
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b2157 7  (SignalChem)
90
SignalChem b2157 7

B2157 7, supplied by SignalChem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/Tau-441+(A152T)+Protein/pmc06450673-25-12-7
Average 90 stars, based on 1 article reviews
b2157 7 - by Bioz Stars, 2026-09
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quattro premier xe triple quadrupole ms spectrometer  (Micromass UK Limited)
90
Micromass UK Limited quattro premier xe triple quadrupole ms spectrometer

Quattro Premier Xe Triple Quadrupole Ms Spectrometer, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sem+and+eds+spectroscopy+layered+elemental+mappings/q+tof+mass+spectrometer/pm22898824-70-6-13
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Image Search Results


( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, HIF-1α and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.

Journal: Nature Communications

Article Title: BCAT1 controls metabolic reprogramming in activated human macrophages and is associated with inflammatory diseases

doi: 10.1038/ncomms16040

Figure Lengend Snippet: ( a ) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n =14 healthy donor hMDMs were used for RNA-seq. ( b ) Chemical structure of ERG240 and leucine. ( c ) Enzymatic activity of recombinant human BCAT1 and BCAT2 in presence of ERG240. ( d ) Relative expression of IRG1 , HIF1A and IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h), and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n =3 healthy donor hMDMs were used in each group. ( e ) Western blot analysis of IRG1, IL-1β, HIF-1α and beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml −1 , 3 h) and LPS+ERG240 treated (LPS, 100 ng ml −1 ; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n =3 healthy donor hMDMs each. ( f ) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml −1 ). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t -test or one-way ANOVA. * P <10 −3 following two-way ANOVA.

Article Snippet: Lipopolysaccharide was purchased from Sigma (LPS; Escherichia coli serotype 0111:B4; Cat No L4391) and the antibodies used in Western Blot analyses are as follows: IL-1β (Cell Signaling Technologies, #12703; D3U3E, dilution 1/1,000), IRG1 (Abcam, ab122624, rabbit polyclonal, dilution, 1/1,000), HIF-1α (NOVUS biological, NB100-449, rabbit polyclonal, dilution 1/2,000), ACTB (Santa Cruz, sc-47778, β-Actin (C4), mouse monoclonal, dilution 1/2,000), BCAT1 (anti Bcat1 raised in rabbit, produced in Susan Hutson’s laboratory , 8382, dilution 1/120,000), TUBB (Cell Signaling Technologies, #2128, rabbit monoclonal, dilution 1/30,000).

Techniques: RNA Sequencing, Derivative Assay, Activity Assay, Recombinant, Expressing, Quantitative RT-PCR, Control, Gene Expression, Western Blot, Gas Chromatography-Mass Spectrometry, Two Tailed Test

Journal: eLife

Article Title: Targeted degradation of aberrant tau in frontotemporal dementia patient-derived neuronal cell models

doi: 10.7554/eLife.45457

Figure Lengend Snippet:

Article Snippet: Peptide, Recombinant Protein , Tau-441(A152T) Protein , SignalChem , Cat. T08-56VN Lot. B2157-7 , Human recombinant protein expressed in E. coli cells, tag-free. Accession no. P10636-8..

Techniques: CRISPR, Knock-Out, Western Blot, Staining, Recombinant, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Lysis, Immunoprecipitation, Protease Inhibitor, Software, Imaging, Mass Spectrometry